Journal: Stem Cell Research & Therapy

Article Title: Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells

doi: 10.1186/s13287-020-01626-6

Figure Lengend Snippet: Primary antibodies

Article Snippet: Nestin , BIO-RAD , AHP1739 , RbP , I, FC, VB , 1:100.

Techniques:

Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Article Title: Neural progenitor cells treated with EPO induce angiogenesis through the production of VEGF

doi: 10.1038/jcbfm.2008.32

Figure Lengend Snippet: Adult SVZ cells are neural progenitor cells. Panels A to D show that the majority of SVZ cells are positive for markers of nestin (A, D) and SOX2 (B, D). Nuclei were stained with 4′,6′-diamidino-2-phenylindole (blue; C, D). Bar = 50 µm.

Article Snippet: The following primary antibodies were used: rabbit anti-nestin (1:100; BD Biosciences) and rabbit anti-SOX2 (1:50; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).

Techniques: Staining

Journal: Cells

Article Title: Glioma Cells Acquire Stem-like Characters by Extrinsic Ribosome Stimuli

doi: 10.3390/cells10112970

Figure Lengend Snippet: Effect of glioma-derived ribosome on stem cell characters in glioma cells. ( A ), Sphere-forming ability in GBM-RICCS induced by the incorporation of GBM ribosome or control (PBS). Bar: 100 µm (each down-scale picture), 200 µm (up-scale picture). ( B ), Stem cell characters were evaluated by sphere formation and the graph showing the number of spheres (>50 µm) cultured for 0, 72, and 120 h. ( C ), Western blotting showing the RPS6, pRPS6, and stem cell markers (Nestin and SOX2) in GBM-RICCS. ( D ), PF4708671 (RPS6 kinase inhibitor) treatment suppressed sphere-forming ability. Bar: 200 µm. * p < 0.05.

Article Snippet: The following antibodies were used to detect anti-gens: rabbit anti-pRPS6 (1:100; Abcam), rabbit anti-Nestin (1:200; Merck), and mouse anti-CD34 (Leica 1:100).

Techniques: Derivative Assay, Cell Culture, Western Blot

Journal: Cells

Article Title: Glioma Cells Acquire Stem-like Characters by Extrinsic Ribosome Stimuli

doi: 10.3390/cells10112970

Figure Lengend Snippet: Possible association between RPS6 phosphorylation and GSC in glioma tissues. ( A ), Images showing higher expression of pRPS6 detected in the perivascular and perinecrosis area compared with the cellular tumor area. Bar: 20 µm. ( B , C ), Images showing double staining with pRPS6, CD34, and Nestin. Bar: 500 µm (each down-scale picture), 50 µm (up-scale picture).

Article Snippet: The following antibodies were used to detect anti-gens: rabbit anti-pRPS6 (1:100; Abcam), rabbit anti-Nestin (1:200; Merck), and mouse anti-CD34 (Leica 1:100).

Techniques: Expressing, Double Staining